Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4601-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 30 mM Ammonium formate

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

5

40

60

3

5.1

0

100

4

6

0

100

5

7

95

5

Flow Rate

500.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S029-UBJ

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

4 mL Water

Load

Load Sample

Wash 1

3 mL 0.1M HCl

Wash 2

3 mL Methanol

Elution 1

3 mL 0.2M Ammonia Solution made up in methanol

Evaporation

Evaporate to dryness

Reconstitute

0 µL Mobile phase