Chlorogenic Acid in Brewed Black Coffee using Kinetex 5u C18 100x4.6mm

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4601-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in MilliQ Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

10

80

20

3

11

80

20

4

11.5

95

5

5

14

95

5

Flow Rate

1.50 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info