Flavonoids Standard using Kinetex 5um C18 150x4.6mm
LC Conditions
Column
Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand
Kinetex
Part No
00F-4601-E0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Trifluoroacetic acid in water
B: 0.1% Trifluoroacetic acid in acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
80
20
2
20
60
40
Flow Rate
1.00 mL/min
Temperature
40°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Quercetin
- 2. Kaempferol
- 3. Isorhamnetin
-OEChem-05081000432D<br><br> 32 34 0 0 0 0 0 0 0999 V2000<br> 6.8671 0.2704 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -1.7296 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6155 -2.7642 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 -2.7296 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3972 0.2945 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 2.7704 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5369 1.7704 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7331 -1.2296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7331 -0.2296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -0.2296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 0.2704 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -1.2296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 -1.7296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6270 -1.7643 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6270 0.3051 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5331 -0.2088 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 1.2704 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5331 -1.2504 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -0.2296 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 1.7704 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 0.2704 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 1.2704 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6199 0.9250 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6720 1.5804 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0688 -1.5625 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -0.8496 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.0396 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -2.3496 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1488 -3.0804 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9353 -0.0134 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8059 3.0804 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.4604 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 9 1 0 0 0 0<br> 1 10 1 0 0 0 0<br> 2 12 1 0 0 0 0<br> 2 28 1 0 0 0 0<br> 3 14 1 0 0 0 0<br> 3 29 1 0 0 0 0<br> 4 13 2 0 0 0 0<br> 5 16 1 0 0 0 0<br> 5 30 1 0 0 0 0<br> 6 20 1 0 0 0 0<br> 6 31 1 0 0 0 0<br> 7 22 1 0 0 0 0<br> 7 32 1 0 0 0 0<br> 8 9 1 0 0 0 0<br> 8 13 1 0 0 0 0<br> 8 14 2 0 0 0 0<br> 9 15 2 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 12 2 0 0 0 0<br> 11 17 2 0 0 0 0<br> 11 19 1 0 0 0 0<br> 12 13 1 0 0 0 0<br> 14 18 1 0 0 0 0<br> 15 16 1 0 0 0 0<br> 15 23 1 0 0 0 0<br> 16 18 2 0 0 0 0<br> 17 20 1 0 0 0 0<br> 17 24 1 0 0 0 0<br> 18 25 1 0 0 0 0<br> 19 21 2 0 0 0 0<br> 19 26 1 0 0 0 0<br> 20 22 2 0 0 0 0<br> 21 22 1 0 0 0 0<br> 21 27 1 0 0 0 0<br>M END
Compound Name
Quercetin
CID
5280343
Molecular Formula
C15H10O7
Molecular Weight
302
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
5
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