OPA Derivitization in Wine of Tyramine and Histamine using Kinetex 2.6u C18
LC Conditions
Column
Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand
Kinetex
Part No
00D-4462-E0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um)
B: 50:50 Acetonitrile/Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
97
3
2
20
20
80
3
20.5
97
3
4
23
97
3
Flow Rate
1.20 mL/min
Temperature
30°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Histamine
- 2. Tyramine
-OEChem-06211003142D<br><br> 17 17 0 0 0 0 0 0 0999 V2000<br> 2.8090 -0.4594 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2633 0.1795 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3090 1.0794 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5691 -0.1807 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6180 0.1284 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3122 0.4885 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3090 1.0794 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.1284 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2780 -0.7281 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0577 -0.5624 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6033 1.0359 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8237 0.8702 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8090 -1.0794 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6734 1.5810 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4103 -0.0632 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7240 0.5943 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3922 -0.4270 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 5 1 0 0 0 0<br> 1 8 1 0 0 0 0<br> 1 13 1 0 0 0 0<br> 2 6 1 0 0 0 0<br> 2 16 1 0 0 0 0<br> 2 17 1 0 0 0 0<br> 3 7 1 0 0 0 0<br> 3 8 2 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 6 1 0 0 0 0<br> 4 9 1 0 0 0 0<br> 4 10 1 0 0 0 0<br> 5 7 2 0 0 0 0<br> 6 11 1 0 0 0 0<br> 6 12 1 0 0 0 0<br> 7 14 1 0 0 0 0<br> 8 15 1 0 0 0 0<br>M END
Compound Name
Histamine
CID
774
Molecular Formula
C5H9N3
Molecular Weight
111
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
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