OPA Derivitization in Wine of Histadine and Histamine using Kinetex 2.6u C18
LC Conditions
Column
Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand
Kinetex
Part No
00D-4462-E0
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um)
B: 50:50 Acetonitrile/Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
97
3
2
20
20
80
3
20.5
97
3
4
23
97
3
Flow Rate
1.20 mL/min
Temperature
30°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Histidine
- 2. Histamine
-OEChem-06211003152D<br><br> 20 20 0 1 0 0 0 0 0999 V2000<br> 7.0064 0.4476 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.4712 -1.1997 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8090 -0.8605 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1043 1.0656 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3090 0.6784 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5691 -0.5817 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3122 0.0874 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 3.6180 -0.2727 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2633 -0.2216 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3090 0.6784 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.2727 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2780 -1.1291 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0577 -0.9634 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7226 0.2790 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8090 -1.4804 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6734 1.1800 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5651 1.4804 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5147 1.2572 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4103 -0.4643 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.5961 0.2560 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 9 1 0 0 0 0<br> 1 20 1 0 0 0 0<br> 2 9 2 0 0 0 0<br> 3 8 1 0 0 0 0<br> 3 11 1 0 0 0 0<br> 3 15 1 0 0 0 0<br> 7 4 1 6 0 0 0<br> 4 17 1 0 0 0 0<br> 4 18 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 11 2 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 12 1 0 0 0 0<br> 6 13 1 0 0 0 0<br> 7 9 1 0 0 0 0<br> 7 14 1 0 0 0 0<br> 8 10 2 0 0 0 0<br> 10 16 1 0 0 0 0<br> 11 19 1 0 0 0 0<br>M END
Compound Name
Histidine
CID
6274
Molecular Formula
C6H9N3O2
Molecular Weight
155
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
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