Whole blood TDP (Vitamin B1) Spiked Standard 400 nmol/L on Gemini 5u C18 150x4.6mm

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 50 x 4.6 mm, Ea

Brand

Gemini

Part No

00B-4435-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 25mM Na2HPO4, 10% Methanol, pH 7.0

B: 25mM Na2HPO4, 70% Methanol, pH 7.0

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

1

87.5

12.5

3

1.5

50

50

4

2.5

10

90

5

2.6

100

0

6

4

100

0

Flow Rate

1.00 mL/min

Temperature

25°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info