Quantification of Acrylamide in Food by LC/MS/MS
LC Conditions
Column
Luna 3 µm C18(2) 100 Å, LC Column 150 x 3 mm, Ea
Brand
Luna
Part No
00F-4251-Y0
Phase Name
C18(2)
Elution Type
Gradient
Mobile Phase
A: Water with 0.001% Formic Acid
B: Methanol with 0.001% Formic Acid
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
90
10
2
10
100
0
3
0
90
10
Flow Rate
250.00 mL/min
Temperature
60°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Acrylamide
-OEChem-06211003152D<br><br> 10 9 0 0 0 0 0 0 0999 V2000<br> 3.7320 -1.0600 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.4400 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -0.0600 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 0.4400 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.0600 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 1.0600 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 0.1300 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 1.0600 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 0.2500 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -0.6800 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 3 2 0 0 0 0<br> 2 3 1 0 0 0 0<br> 2 7 1 0 0 0 0<br> 2 8 1 0 0 0 0<br> 3 4 1 0 0 0 0<br> 4 5 2 0 0 0 0<br> 4 6 1 0 0 0 0<br> 5 9 1 0 0 0 0<br> 5 10 1 0 0 0 0<br>M END
Compound Name
Acrylamide
CID
6579
Molecular Formula
C3H5NO
Molecular Weight
71
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1
Similar Applications
Reversed Phase
Quantification of Acrylamide in Food by LC/MS/MS
Luna 3 µm C18(2) 100 Å, LC Column 150 x 3 mm, Ea
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