Nicotinic acid / Nicotinamide (10 ng/mL) in Human Plasma by Impact on Gemini 3µm C18 100x4.6mm

Nicotinic acid / Nicotinamide (10 ng/mL) in Human Plasma by Impact on Gemini 3µm C18 100x4.6mm
LC Conditions
Column

Gemini 3 µm C18 110 Å, LC Column 100 x 4.6 mm, Ea

Brand

Gemini

Part No

00D-4439-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid

B: Methanol 100%

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

2.5

10

90

3

2.6

90

10

4

4

90

10

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Electrospray Mass Spec (ESMS) @ (22°C)

Detection Info
Sample Preparation Details
Description

Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk

Part No

CE0-7565

Load

Load Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase

Order Items Used in This Application

Gemini 3 µm C18 110 Å, LC Column 100 x 4.6 mm, Ea
00D-4439-E0

Gemini 3 µm C18 110 Å, LC Column 100 x 4.6 mm, Ea

View Product

Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
CE0-7565

Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk

View Product

  • 1. Nicotinamide
  • 2. Nicotinic acid
-OEChem-06211003142D<br><br> 15 15  0     0  0  0  0  0  0999 V2000<br>    2.0000    1.4400    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -1.5600    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320    1.4400    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -0.0600    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000   -0.5600    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -0.5600    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000   -1.5600    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660    0.9400    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -2.0600    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631   -0.2500    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.2690   -0.2500    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631   -1.8700    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -2.6800    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320    2.0600    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.2690    1.1300    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1  8  2  0  0  0  0<br>  2  6  1  0  0  0  0<br>  2  9  2  0  0  0  0<br>  3  8  1  0  0  0  0<br>  3 14  1  0  0  0  0<br>  3 15  1  0  0  0  0<br>  4  5  1  0  0  0  0<br>  4  6  2  0  0  0  0<br>  4  8  1  0  0  0  0<br>  5  7  2  0  0  0  0<br>  5 10  1  0  0  0  0<br>  6 11  1  0  0  0  0<br>  7  9  1  0  0  0  0<br>  7 12  1  0  0  0  0<br>  9 13  1  0  0  0  0<br>M  END
Compound Name

Nicotinamide

CID

936

Molecular Formula

C6H6N2O

Molecular Weight

122

No. Hydrogen Bond Acceptors

2

No. Hydrogen Bond Donors

1

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