Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC (#20715)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

12

53

47

3

12.1

2

98

4

15

2

98

5

15.1

95

5

6

22

95

5

Flow Rate

0.50 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info