Azo Dyes by LC/MS/MS

LC Conditions

Column

Luna 5 µm C8 100 Å, LC Column 150 x 2 mm, Ea

Brand

Luna

Part No

00F-4040-B0

Phase Name

C8

Elution Type

Gradient

Mobile Phase

A: Water with 0.2 % Formic acid and 2 mM Ammonium formate

B: Water/Acetonitrile(10:90) with 0.2 % Formic acid and 2 mM Ammonium formate

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

15

0

100

3

29

0

100

4

30

80

20

Flow Rate

300.00 mL/min

Temperature

30°C

Detection

Tandem Mass Spec (MS-MS) @ (450°C)

Detection Info