Rapid analysis of BSA digest using Kinetex 1.3u C18 50x2.1mm

LC Conditions

Column

Kinetex 1.3 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4515-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

1

95

5

3

11

5

95

4

11.5

5

95

5

11.6

95

5

Flow Rate

0.50 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info