Reduced Human Ig-G1 on Aeris: LC/MS Fast Separation

LC Conditions

Column

Aeris 3.6 µm WIDEPORE XB-C8 200 Å, LC Column 100 x 2.1 mm, Ea

Brand

Aeris

Part No

00D-4481-AN

Phase Name

WIDEPORE XB-C8

Elution Type

Gradient

Mobile Phase

A: 0.2% Formic Acid in Water

B: IPA/AcCN/0.2% formic acid in water 80:10:10

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

10

5

95

3

14

5

95

4

14.01

95

5

Flow Rate

0.40 mL/min

Temperature

70°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info