Reduced Human Ig-G1 on Aeris: LC-UV Separation

LC Conditions

Column

Aeris 3.6 µm WIDEPORE XB-C8 200 Å, LC Column 100 x 2.1 mm, Ea

Brand

Aeris

Part No

00D-4481-AN

Phase Name

WIDEPORE XB-C8

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: IPA/AcCN/0.1% TFA in water 80:10:10

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

5

60

40

3

15

28

72

4

19

24

76

5

19.01

3

97

6

20

3

97

Flow Rate

0.40 mL/min

Temperature

70°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info