Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_2.5nmol vs Blank

LC Conditions

Column

Gemini 3 µm NX-C18 110 Å, LC Column 100 x 3 mm, Ea

Brand

Gemini

Part No

00D-4453-Y0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 25nM Na2HPO4, 10% methanol

B: 25mM Na2HPO4, 70% methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

3

97

2

0.25

25

75

3

0.75

25

75

4

3

35

65

5

4

100

0

6

5

100

0

7

5.1

3

97

8

8

3

97

Flow Rate

0.75 mL/min

Temperature

22°C

Detection

Fluorescence (FLUOR) @ (22°C)

Detection Info

Sample Preparation Details

Description

Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk

Part No

CE0-7565

Load

Load Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase