EPA 554: Carbonyl Compounds in Drinking Water

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4601-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

2

15

0

100

3

20

0

100

Flow Rate

2.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata C18-E (55 µm, 70 Å), 500 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S001-HCH

Pretreatment Note

After elution Q.S. to 10 mL with ethanol before injection

Condition 1

10 mL 40 mM citrate buffer pH 3.0 at 2 mL/min

Load

Load 100.00 mL Sample diluted in Derivatized sample

Elution 1

10 mL Ethanol at 1 mL/min

Evaporation

Evaporate to dryness