EPA 532 on Kinetex 5 µm C18 150 x 4.6

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4601-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 25 mM Phosphate buffer pH 2.4

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

60

40

2

9.5

60

40

3

10

50

50

4

14

40

60

5

15

60

40

Flow Rate

1.50 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info