nspiked Ground Meat Extract for antibiotic analysis on Kinetex XB-C18

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4496-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 0.1% Formic Acid in 50:50 MeOH:ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

1

95

5

3

4

50

50

4

6

5

95

5

7.5

5

95

6

7.51

95

5

7

10

95

5

Flow Rate

450.00 mL/min

Temperature

50°C

Detection

Tandem Mass Spec (MS-MS) @ (1°C)

Detection Info