Improved Extraction of Cannabinoids from Oral Fluid THC-OH Curve

LC Conditions

Column

Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4495-E0

Phase Name

Phenyl-Hexyl

Elution Type

Gradient

Mobile Phase

A: 10 mM Ammonium Formate

B: 0.1% Formic Acid in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

4

0

100

3

6

0

100

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-Drug B 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S128-TBJ

Condition 1

1 mL Methanol

Condition 2

1 mL Acetonitrile: 100 mM sodium acetate buffer, pH 5.0 (30/70)

Load

Load Sample

Wash 1

1 mL 100 mM sodium acetate buffer, pH 5.0

Wash 2

1 mL Acetonitrile: 100 mM sodium acetate buffer, pH 5.0 (30/70)

Dry

5 min. under vacuum at 10 inches Hg

Elution 1

0.5 mL Ethyl acetate:Isopropanol (85/15)

Elution 2

0.5 mL Ethyl acetate:Isopropanol (85/15)

Evaporation

Evaporate to dryness

Reconstitute

500 µL mobile phase (A/B=55/45)