Linearity of Cyclosporine A from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm

LC Conditions
Column

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Aeris

Part No

00B-4507-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 5 mM ammonium formate

B: 5mM ammonium formate in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

1

5

95

3

2

5

95

4

2.01

65

35

5

4

65

35

Flow Rate

0.70 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Order Items Used in This Application

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
00B-4507-AN

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

View Product

  • 1. Cyclosporin A
-OEChem-05081000432D<br><br>196196  0     1  0  0  0  0  0999 V2000<br>    9.1236   -5.6277    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.7372   -4.9728    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.8725   -3.3565    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.6361   -0.3017    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.2259   -4.6375    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.4683    3.2216    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6840    6.7383    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.4532   -2.4571    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.1312    4.3042    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.1476    7.4058    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.2996    0.8763    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.4222    6.0949    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.3963   -3.2509    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.5525   -4.0859    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.3372   -1.0110    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.7590    1.9227    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.7087   -3.2509    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.0706    6.2211    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.5772    4.6187    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.3460    1.9227    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.7678   -1.0110    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.5278    4.6187    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.0344    6.2211    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.5075   -3.7091    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   13.8372   -1.8770    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    8.5570   -3.9909    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   11.8791   -4.6375    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.3678   -4.9728    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   14.6518   -2.4571    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.7129   -5.7285    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   10.5480   -3.9909    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.1824   -2.6328    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.8066    0.9238    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   15.6473   -2.5521    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.6643   -0.0660    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.8055    0.8763    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.5976   -3.7091    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.0400   -6.6736    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.8690   -4.7798    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.2610   -5.4236    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.6943    1.3345    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.9370   -4.0922    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.7310   -5.5393    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.5525   -5.0859    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.2469   -1.4264    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.0627   -3.4618    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.2273   -1.7375    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.9227   -2.6328    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    6.3010    5.3088    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   14.5233    2.8945    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.0525    6.4104    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   17.5356    0.7938    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.7419    2.3334    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5818    2.8945    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    7.3851   -7.4293    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.7489    2.0650    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.6828    6.0949    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.9575    7.4058    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.1079    3.8042    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    3.6368    3.2216    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.2326   -3.3565    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.1422    5.8494    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.3729    8.3155    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.2678   -1.8770    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.6548    3.0324    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.9972    3.8042    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.0525    6.4104    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.4407   -0.0660    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.6930    5.9525    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0545    7.0232    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.2984    0.9238    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.9628    5.8494    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>   14.9739    4.3042    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.7122   -8.3743    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.7889    7.1806    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.5143   -4.3160    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.7928    9.1300    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.3684    8.4105    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.8041    5.3088    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000    3.7882    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.3278    2.0873    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.3562    2.0650    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.8582   -1.4264    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.4210    6.7383    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.0573   -9.1300    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.3039   -4.0062    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.6678   -1.6963    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.0664   -4.6713    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.2655   -4.5493    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.1710   -4.6948    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.1411   -2.8086    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.8685   -1.8762    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.4349   -4.9252    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.4077    1.5249    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.2876   -3.0572    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.5660    0.3044    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.6737    1.4821    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.4310   -7.1548    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.5818   -6.3721    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.7807   -5.3935    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.4827   -4.8680    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.9572   -4.1661    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.7736   -5.0403    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.8777   -5.9109    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.7483   -5.8068    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   17.2454    1.6186    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.4154   -4.4274    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.2722   -4.6137    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.4585   -3.7570    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.8483   -4.9305    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.1222   -5.4220    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6137   -6.1481    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.9325   -5.0859    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.5525   -5.7059    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.1725   -5.0859    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.9893   -1.9904    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.8108   -1.6840    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.5044   -0.8624    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.4987   -3.7193    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.3203   -4.0257    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.6267   -3.2042    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.7324   -2.0972    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.5870   -1.2325    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.7223   -1.3779    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.3735   -3.7725    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.9833   -3.4806    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.0062   -6.2364    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4531    5.3694    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.3721    6.9198    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.8357    2.4872    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   17.2004    0.2723    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   18.0572    0.4587    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   17.8708    1.3154    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   17.3612    2.3039    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.7714    2.9527    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.1226    2.3629    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.7763   -7.3119    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.8371    1.4513    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   16.3625    2.1533    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.6606    2.6787    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.2996    6.5822    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.5156    7.8408    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.5689    7.3030    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.2885    4.6347    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.0277    3.7028    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.5925    2.6032    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.8650   -2.8572    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.6771   -3.6318    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.7557    8.2565    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0460    2.9150    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.1087    5.0248    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.8517    0.1275    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.0433    0.0802    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   13.9964    5.0248    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.6048    6.5662    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.0793    5.8643    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.7812    5.3389    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6301    6.7928    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.2849    7.5988    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4789    7.2537    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.6658    6.6459    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.2839    3.7672    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   15.5108    4.6142    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   14.6639    4.8411    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.3210   -8.4916    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.3837    7.3553    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.6142    7.7755    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.1940    7.0059    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.9194   -4.4906    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.6890   -4.9109    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.1092   -4.1413    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.2091    6.8160    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    9.2979    9.4897    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.4332    9.6351    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    8.2878    8.7704    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.4273    7.7933    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.9855    8.4695    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   10.3094    9.0277    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.4686    4.1941    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.5940    4.2568    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.5314    3.3822    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.7419    2.2900    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.1251    1.5014    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.9137    1.8846    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.4444    2.6787    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.7425    2.1533    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.2680    1.4513    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.6006   -0.8624    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.2942   -1.6840    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.1157   -1.9904    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.9721    6.4542    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   12.7051    7.2894    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>   11.8700    7.0224    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.5888   -8.7240    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6513   -9.5986    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    7.5259   -9.5361    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1 28  1  0  0  0  0<br>  1127  1  0  0  0  0<br>  2 31  2  0  0  0  0<br>  3 32  2  0  0  0  0<br>  4 35  2  0  0  0  0<br>  5 37  2  0  0  0  0<br>  6 50  2  0  0  0  0<br>  7 62  2  0  0  0  0<br>  8 64  2  0  0  0  0<br>  9 66  2  0  0  0  0<br> 10 67  2  0  0  0  0<br> 11 71  2  0  0  0  0<br> 12 79  2  0  0  0  0<br> 13 24  1  0  0  0  0<br> 13 32  1  0  0  0  0<br> 13 42  1  0  0  0  0<br> 14 26  1  0  0  0  0<br> 14 31  1  0  0  0  0<br> 14 44  1  0  0  0  0<br> 15 25  1  0  0  0  0<br> 15 35  1  0  0  0  0<br> 15 45  1  0  0  0  0<br> 16 33  1  0  0  0  0<br> 16 50  1  0  0  0  0<br> 16 56  1  0  0  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Compound Name

Cyclosporin A

CID

5280754

Molecular Formula

C62H111N11O12

Molecular Weight

1202

No. Hydrogen Bond Acceptors

12

No. Hydrogen Bond Donors

5

Similar Applications

Reversed Phase

Linearity of Cyclosporine A from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

22100

Reversed Phase

Analysis of Immunosuppresant from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

22098