Linearity of Sirolimus from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm

LC Conditions

Column

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Aeris

Part No

00B-4507-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 5 mM ammonium formate

B: 5mM ammonium formate in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

1

5

95

3

2

5

95

4

2.01

65

35

5

4

65

35

Flow Rate

0.70 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info