Linearity of Tacrolimus from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm
LC Conditions
Column
Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Aeris
Part No
00B-4507-AN
Phase Name
PEPTIDE XB-C18
Elution Type
Gradient
Mobile Phase
A: 5 mM ammonium formate
B: 5mM ammonium formate in Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
65
35
2
1
5
95
3
2
5
95
4
2.01
65
35
5
4
65
35
Flow Rate
0.70 mL/min
Temperature
22°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Tacrolimus
-OEChem-05271007432D<br><br>126129 0 1 0 0 0 0 0999 V2000<br> 7.6897 -2.9934 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.4627 -4.4461 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3150 -3.7595 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6897 -1.9934 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3895 -0.6097 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2349 4.7171 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2124 -3.6057 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.9670 3.7171 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.9671 -2.3111 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2608 2.5659 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1216 -1.6096 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1434 3.0457 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9876 -1.1097 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8237 -2.4934 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.5675 -3.5091 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.8120 -3.5091 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.6897 -4.0204 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5558 -2.4934 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.9577 -2.9934 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.0916 -2.4934 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0916 -1.4934 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.9168 -4.4462 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3689 2.2171 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 9.4218 -2.9934 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3689 3.2171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2349 1.7171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2349 3.7171 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 11.1009 3.2171 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 11.1009 2.2171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1216 -0.6097 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.2256 -0.9934 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9876 -2.1097 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3895 0.3903 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 9.1216 0.3903 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.8536 -0.6097 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5029 1.7171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5235 0.8903 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 9.9876 0.8903 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.8536 0.3903 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3292 0.7323 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9577 -0.9934 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5235 1.8903 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.2556 -1.1097 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2256 0.0066 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0996 -5.2171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3301 -3.5858 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.8164 2.5974 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5235 -0.1097 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5916 1.3727 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 9.2102 -0.0069 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0916 0.5066 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8505 2.3386 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3596 0.5066 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6002 1.2421 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.1009 5.2171 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.9914 2.0355 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.9050 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.4030 -1.7548 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1770 -3.3956 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2024 -3.3956 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2906 -4.4949 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0889 -4.4949 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3562 -3.4683 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4811 -2.3857 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8796 -3.0760 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0916 -0.8734 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3358 -4.6627 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1333 -5.0271 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4977 -4.2296 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3689 1.5971 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1568 3.7997 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.7583 3.1095 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.8364 1.2422 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.6334 1.2422 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.6980 4.0271 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6379 2.9071 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.3130 1.6345 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.7115 2.3248 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3855 -0.1847 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6150 -0.8857 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0136 -1.5760 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8974 0.0347 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9095 0.9729 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5110 0.2826 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6897 -1.3734 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.0657 -1.1923 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4642 -0.5020 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0279 2.1157 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9035 0.8903 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.3861 1.3652 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5891 1.3652 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4642 0.2826 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.0657 0.9729 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2677 -1.5303 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.4946 -0.6834 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6477 -0.4564 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0910 1.6406 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6216 -5.6120 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4945 -5.6951 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.5776 -4.8223 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3243 2.9530 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5544 3.1593 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4378 -2.9752 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7195 -3.4781 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2224 -4.1964 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9035 -0.1097 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5235 -0.7297 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1435 -0.1097 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1829 1.8388 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8116 -0.4818 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.6851 -0.4054 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.6087 0.4681 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5301 0.0682 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 12.5039 3.4071 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6696 1.0436 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8227 0.8166 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0496 -0.0303 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.0503 0.9559 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7866 0.6508 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.4109 4.6802 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11.6379 5.5271 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.7909 5.7541 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1266 3.1712 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2287 2.6083 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6226 2.3968 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.7627 1.3322 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 14 1 0 0 0 0<br> 1 18 1 0 0 0 0<br> 16 2 1 6 0 0 0<br> 2 45 1 0 0 0 0<br> 19 3 1 1 0 0 0<br> 3 46 1 0 0 0 0<br> 18 4 1 6 0 0 0<br> 4 85 1 0 0 0 0<br> 5 33 1 0 0 0 0<br> 5 43 1 0 0 0 0<br> 27 6 1 1 0 0 0<br> 6 55 1 0 0 0 0<br> 7 24 2 0 0 0 0<br> 28 8 1 6 0 0 0<br> 8114 1 0 0 0 0<br> 9 32 2 0 0 0 0<br> 42 10 1 6 0 0 0<br> 10123 1 0 0 0 0<br> 11 43 2 0 0 0 0<br> 12 52 2 0 0 0 0<br> 13 30 1 0 0 0 0<br> 13 32 1 0 0 0 0<br> 13 35 1 0 0 0 0<br> 14 16 1 0 0 0 0<br> 14 19 1 0 0 0 0<br> 14 58 1 6 0 0 0<br> 15 17 1 0 0 0 0<br> 15 18 1 0 0 0 0<br> 15 22 1 6 0 0 0<br> 15 59 1 0 0 0 0<br> 16 17 1 0 0 0 0<br> 16 60 1 0 0 0 0<br> 17 61 1 0 0 0 0<br> 17 62 1 0 0 0 0<br> 18 24 1 0 0 0 0<br> 19 20 1 0 0 0 0<br> 19 63 1 0 0 0 0<br> 20 21 1 0 0 0 0<br> 20 64 1 0 0 0 0<br> 20 65 1 0 0 0 0<br> 21 31 1 0 0 0 0<br> 21 41 1 6 0 0 0<br> 21 66 1 0 0 0 0<br> 22 67 1 0 0 0 0<br> 22 68 1 0 0 0 0<br> 22 69 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 23 26 1 0 0 0 0<br> 23 36 1 1 0 0 0<br> 23 70 1 0 0 0 0<br> 24 32 1 0 0 0 0<br> 25 27 1 0 0 0 0<br> 25 71 1 0 0 0 0<br> 25 72 1 0 0 0 0<br> 26 29 1 0 0 0 0<br> 26 73 1 0 0 0 0<br> 26 74 1 0 0 0 0<br> 27 28 1 0 0 0 0<br> 27 75 1 0 0 0 0<br> 28 29 1 0 0 0 0<br> 28 76 1 0 0 0 0<br> 29 77 1 0 0 0 0<br> 29 78 1 0 0 0 0<br> 30 34 1 0 0 0 0<br> 30 43 1 0 0 0 0<br> 30 79 1 1 0 0 0<br> 31 44 1 0 0 0 0<br> 31 80 1 0 0 0 0<br> 31 81 1 0 0 0 0<br> 33 37 1 0 0 0 0<br> 33 40 1 1 0 0 0<br> 33 82 1 0 0 0 0<br> 34 38 1 0 0 0 0<br> 34 83 1 0 0 0 0<br> 34 84 1 0 0 0 0<br> 35 39 1 0 0 0 0<br> 35 86 1 0 0 0 0<br> 35 87 1 0 0 0 0<br> 36 40 2 0 0 0 0<br> 36 88 1 0 0 0 0<br> 37 42 1 0 0 0 0<br> 37 48 1 6 0 0 0<br> 37 89 1 0 0 0 0<br> 38 39 1 0 0 0 0<br> 38 90 1 0 0 0 0<br> 38 91 1 0 0 0 0<br> 39 92 1 0 0 0 0<br> 39 93 1 0 0 0 0<br> 40 50 1 0 0 0 0<br> 41 94 1 0 0 0 0<br> 41 95 1 0 0 0 0<br> 41 96 1 0 0 0 0<br> 42 47 1 0 0 0 0<br> 42 97 1 0 0 0 0<br> 44 51 2 0 0 0 0<br> 44 53 1 0 0 0 0<br> 45 98 1 0 0 0 0<br> 45 99 1 0 0 0 0<br> 45100 1 0 0 0 0<br> 46103 1 0 0 0 0<br> 46104 1 0 0 0 0<br> 46105 1 0 0 0 0<br> 47 52 1 0 0 0 0<br> 47101 1 0 0 0 0<br> 47102 1 0 0 0 0<br> 48106 1 0 0 0 0<br> 48107 1 0 0 0 0<br> 48108 1 0 0 0 0<br> 49 51 1 0 0 0 0<br> 49 52 1 0 0 0 0<br> 49 54 1 1 0 0 0<br> 49109 1 0 0 0 0<br> 50110 1 0 0 0 0<br> 50111 1 0 0 0 0<br> 50112 1 0 0 0 0<br> 51113 1 0 0 0 0<br> 53115 1 0 0 0 0<br> 53116 1 0 0 0 0<br> 53117 1 0 0 0 0<br> 54 56 1 0 0 0 0<br> 54118 1 0 0 0 0<br> 54119 1 0 0 0 0<br> 55120 1 0 0 0 0<br> 55121 1 0 0 0 0<br> 55122 1 0 0 0 0<br> 56 57 2 0 0 0 0<br> 56124 1 0 0 0 0<br> 57125 1 0 0 0 0<br> 57126 1 0 0 0 0<br>M END
Compound Name
Tacrolimus
CID
445643
Molecular Formula
C44H69NO12
Molecular Weight
804
No. Hydrogen Bond Acceptors
12
No. Hydrogen Bond Donors
3
Similar Applications
Reversed Phase
Tacrolimus Standard solution 30 µg/ml. USP Organic impurities Procedure-2
Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
27997
Reversed Phase
Tacrolimus standard solution 30µg/ml. USP-Organic impurities
Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
27990
Reversed Phase
Tacrolimus Standard solution five replicated injections 3mg/ml. USP-Assay
Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
27969
Reversed Phase
Tacrolimus system suitability solution for USP- Assay
Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
27967
Reversed Phase
Tacrolimus Standard solution 3mg/mL - USP- Assay
Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
27965
Reversed Phase
Tacrolimus system suitability solution. USP-Assay
Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
27958
Reversed Phase
Linearity of Tacrolimus from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm
Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
22103
Reversed Phase
Analysis of Immunosuppresant from Whole blood Using PPT by LCMS using Aeris 3.6um, XB-C18, 50x2.1mm
Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
22098