THC and metabolites by LCMS using Kinetex 5u Biphenyl 50x2.1mm

LC Conditions

Column

Kinetex 5 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4627-AN

Phase Name

Biphenyl

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid

B: Methanol with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

2

2.5

5

95

3

4

5

95

4

4.1

50

50

5

5.5

50

50

Flow Rate

0.40 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info