Bivalirudin crude with 0.1% TFA + ACN on knx xb-c18 2.6um 50x4.6

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4496-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

2.75

90

10

3

22.5

80

20

4

25

80

20

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info