IgG1 (Ascites Fluid) Digest in TFA: Aeris PEPTIDE 1.7u XB-C18 150 x 2.1 mm

LC Conditions

Column

Aeris 1.7 µm PEPTIDE XB-C18 100 Å, LC Column 150 x 2.1 mm, Ea

Brand

Aeris

Part No

00F-4506-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA/2% AcCN/in water

B: 0.09%TFA in 90%AcCN/10% water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

2

97

3

3

49

50

50

4

54

5

95

Flow Rate

0.40 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info