Mycotoxins extracted from corn using roQ by LC/MS/MS (Negative mode)

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4496-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 5 mM ammonium acetate w/ 0.5% acetic acid

B: 5 mM ammonium acetate in methanol w/ 0.5% acetic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

2

95

5

3

5

20

80

4

5.2

2

98

5

8

2

98

Flow Rate

0.45 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

roQ extraction: 1) 5 g of corn is extracted using KS0-8909 with 10 mL of water and 10 mL of ACN with 1.0 % formic acid. 2. KSO-8924 is used for dSPE clean up.