Opiates at low pH using Kinetex 5u C18 50 x 2.1 mm

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4601-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% Formic acid

B: Methanol with 0.1% Formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

0.5

90

10

3

2

75

25

4

4.5

20

80

5

4.51

15

85

6

5.5

15

85

7

5.51

90

10

8

7

90

10

Flow Rate

500.00 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info