Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea

Brand

Kinetex

Part No

00D-4633-Y0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 20 mM Ammonium bicarbonate pH 8.2

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

3

10

90

3

3.1

90

10

4

5

90

10

5

5

95

5

Flow Rate

0.75 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info