Phospholipid Removal Novum EtoAC Elution(Zoomed)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

40

60

2

0.5

5

95

3

15.5

5

95

4

15.51

40

60

5

19.5

40

60

Flow Rate

400.00 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (425°C)

Detection Info

Sample Notes

Extracted sample was prepped with Phenomenex Novum SLE Plate (400 uL sample Load) Step 1: 400 uL Dilute Plasma (50/50) to the plate. Step 2: Elute with 1800 uL of EtOAc (two steps, 900 uL each).

Sample Preparation Details

Load

Load Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase