Customer high pH study using Kinetex 5u EVO C18 150x4.6mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4633-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Ethanolamine (pH ~10.5)

B: Acetonitrile with 0.1% Ethanolamine

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

5

10

90

Flow Rate

1.50 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info