SOFT 2014 Fig1 Representative Chromatogram of Drug Panel

LC Conditions

Column

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea

Brand

Kinetex

Part No

00B-4622-Y0

Phase Name

Biphenyl

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% formic acid

B: Methanol with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

2.5

0

100

3

3.5

0

100

4

3.6

90

10

5

5

90

10

Flow Rate

0.70 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info