Typical Chromatogram of 6 Cannabinoids

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 150 x 3 mm, Ea

Brand

Kinetex

Part No

00F-4496-Y0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 5 mM Phosphate Buffer, pH 2.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

25

75

2

3

5

95

3

6

5

95

Flow Rate

0.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (40°C)

Detection Info