LCMS of Vitamin D epimers on Kinetex 2.6u F5 100x4.6mm

LC Conditions

Column

Kinetex 2.6 µm F5 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4723-E0

Phase Name

F5

Elution Type

Isocratic

Mobile Phase

A: Water with 0.1% formic acid

B: Methanol with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

15

85

Flow Rate

0.75 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info