Acrylamide from Coffee by SLE and LC/MS/MS

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 50 x 2 mm, Ea

Brand

Synergi

Part No

00B-4375-B0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 0.1% FA in Water

B: 0.1%FA in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

0.6

100

0

3

0.85

0

100

4

3

0

100

5

3.01

100

0

6

5

100

0

Flow Rate

0.30 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info