25-OH-Vitamin D3 in Human Serum using Novum SLE and Kinetex 2.6um F5 100 x 4.6mm

LC Conditions

Column

Kinetex 2.6 µm F5 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4723-E0

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: 0.1% FA in Water

B: 0.1%FA in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

15

85

2

5

15

85

3

5.01

0

100

4

6.5

0

100

5

6.51

15

85

6

8

15

85

Flow Rate

0.75 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info