Ephedrine Alkaloids in Ephedra Extract by HPLC

LC Conditions

Column

Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand

Synergi

Part No

00F-4336-E0

Phase Name

Polar-RP

Elution Type

Isocratic

Mobile Phase

A: Methanol/100 mM KH2PO4 3:97

Gradient Profile

Step No.

Time(min)

%A

%B

1

20

100

0

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info