5-Hydroxytryptophan in Dietary Supplement Capsules by HPLC

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: 95:5:0.2 WaterH3PO4 + 10 mM HSA

B: 50:50:0.2 WaterH3PO4 + 10 mM HSA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

30

0

100

Flow Rate

1.50 mL/min

Temperature

25°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info