Low levels of B12 analysis from Vitamin pill formulation on Kinetex 5u EVO 150x4.6mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4633-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 3g Sodium 1-Hexanesulfonate Monohydrate with 0.4mL TEA and 15mL Acetic Acid with 1985mL Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

10

2

98

3

12

2

98

4

12.1

95

5

5

15

95

5

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info