Alpha Beta Gamma Delta Tocopherols and Tocotrienols on Kinetex 2.6u F5 150x4.6mm

LC Conditions

Column

Kinetex 2.6 µm F5 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4723-E0

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

15

85

2

1

15

85

3

15

0

100

4

17

0

100

5

18

15

85

6

23

15

85

Flow Rate

1.20 mL/min

Temperature

42°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

This was ran on an Agilent 1100 optimized system.