Peptide map on Kinetex 2.6 µm EVO C18 (Phosphorylase B)

LC Conditions

Column

Kinetex 2.6 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, EA

Brand

Kinetex

Part No

00F-4725-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA/2% ACN in Water

B: 0.1% TFA/ ACN (10:90)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

68

30

70

Flow Rate

1.50 mL/min

Temperature

60°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Forced degradation protocol: Reconstitute 1 mg of cefaclor neat standard with 1.0 mL of 1 M HCl. Heat the solution at 60 ºC for 6 hours then neutralize with 1.0 mL of 1 M NaOH. Refrigerate until ready to inject.