Amino Acid Analysis using OPA on Kinetex 5u EVO C18 150x4.6mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4633-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium Phosphate pH 7.2

B: 50:50 Acetonitrile:Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

20

40

60

3

23

97

3

Flow Rate

1.00 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info