Bupropion related compounds on Kinetex 5u EVO C18 150x4.6mm

LC Conditions

Column

Kinetex 5 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4633-E0

Phase Name

EVO C18

Elution Type

Gradient

Mobile Phase

A: 5mM Ammonium Bicarbonate pH 9.0

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

2

10

20

80

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-CW 33 µm Polymeric Weak Cation, 60 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S035-UBJ

Condition 1

3 mL Metahnol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Load

Load 1.00 mL Sample diluted in 100mM Sodium Phosphate pH 6.8

Wash 1

3 mL 100mM Sodium Phosphate pH 6.8 at 1 mL/min

Dry

5 minutes

Elution 1

2 mL 5% Formic Acid in Acetonitrile at 0.5 mL/min

Evaporation

Evaporate to dryness

Reconstitute

500 µL 50:50 5mM Ammonium Bicarbonate pH 9.0 and Methanol