Vitamin A and E from infant formula (Enfamil) on Luna NH2 by UV

LC Conditions

Column

Luna 3 µm NH2 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4377-E0

Phase Name

NH2

Elution Type

Gradient

Mobile Phase

A: Hexane

B: 50 % MTBE in Hexane

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

3

100

0

3

8

0

100

4

12

0

100

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Sample prepared following AOAC official method 2012.10 using "Enfamil" infant formula liquid