Vitamin D tablet assay (standards) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6

LC Conditions

Column

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4622-E0

Phase Name

Biphenyl

Elution Type

Isocratic

Mobile Phase

A: 0.1% TFA

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

35

65

Flow Rate

1.75 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info