Hormones Extracted from Sediment using QuEChERS by LCMSMS

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4496-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: DI Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

1

80

20

3

7.25

40

60

4

8

20

80

5

9.5

20

80

6

9.55

85

15

7

14

85

15

Flow Rate

500.00 μL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info