Tryptic digest by LCMS using Luna Omega Polar C18 100x2.1mm

LC Conditions

Column

Luna Omega 1.6µm Polar C18 100Å, LC Column 100 x 2.1 mm, EA

Brand

Luna Omega

Part No

00D-4748-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% formic acid

B: Acetonitrile with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

50

50

50

3

50.1

97

3

Flow Rate

400.00 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (450°C)

Detection Info