THC-COOH comparison using Beta-Gone and Dilute-n-Shoot

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part Number

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% Formic acid

B: Acetonitrile with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

3

5

95

3

4

5

95

4

4.1

95

5

Flow Rate

500.00 mL/min

Temperature

0°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Preparation Details

Load

Load 333.00 µL Sample

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase