Extracted Ion Chromatograms for EtG/EtS and their Deuterated Internal Standards, 500ng/mL

LC Conditions

Column

Luna Omega 1.6µm Polar C18 100Å, LC Column 100 x 2.1 mm, EA

Brand

Luna Omega

Part No

00D-4748-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 10 mM ammonium formate in 0.1% formic acid, pH unadjusted (~3.2)

B: 50:50 MeOH/ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

100

0

2

1

100

0

3

2

75

25

4

3

5

95

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (40°C)

Detection Info