PPCPs on a Kinetex 2.6 µm C18 by LCMSMS (+ve)

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: 0.1% Formic acid in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

4

0

100

3

5

0

100

4

5.01

90

10

5

8

90

10

Flow Rate

400.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info