EtG/EtS in Urine using Synergi 2.5µm Hydro-RP, 100x3mm (3 min)

LC Conditions

Column

Synergi 2.5 µm Hydro-RP 100 Å, LC Column 100 x 3 mm, Ea

Brand

Synergi

Part No

00D-4387-Y0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 10mM ammonium formate + 0.1% formic acid in water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

1.5

5

95

3

2

5

95

4

2.01

100

0

5

3

100

0

Flow Rate

0.70 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (550°C)

Detection Info