Pain Management Booklet - SAMHSA-5

LC Conditions

Column

Kinetex 5 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea

Brand

Kinetex

Part No

00B-4627-Y0

Phase Name

Biphenyl

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid in water

B: 0.1% formic acid in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

1.5

2

98

3

2.5

2

98

4

2.51

95

5

5

4

95

5

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info